Principles of Molecular Biology Protein Annotation Project Assignment

Principles of Molecular Biology Protein Annotation Project Assignment

• exploit knowledge of the fundamental principles of molecular biology to analyze contributions of DNA and proteins to human physiology and behavior
• identify, select, and query the appropriate database to best answer the bioinformatics question at hand, and effectively and accurately interpret the outcome

For the protein annotation project, you will use the protein coded by the gene in the gene annotation project, if it was noncoding gene, choose any protein of interest to annotate. The following tasks must be completed and must provide screenshots

• Provide the protein sequence from the provided gene sequence and state protein name, gene name and amino acid length. Include accession ID or protein ID from UniProt, or NCBI
• identify the protein function
• protein family, conserved domains: name and location by amino acid number
• gene ontology (GO): Provide 2 GOs per each one of these sections where available. Sections: Molecular function, Biological process and cellar component. Include GO ID, name, and definition
• computational analysis of protein-protein interactions: total number of interactors and at least three names and protein ids of the interactors where available, if there is none, choose 3 other unique features of your protein not on the list

Do not cut and paste your database results to report your findings. Write two to three paragraphs reporting the information you have obtained about this protein. Imagine this is a report you will present to an employer or a group of researchers interested in this protein.

Content	Excellent	Very Good	Good	Fair	Poor
Points	4	3	2	1	0
Protein Identification	correct identification of protein name, gene name, sequence, protein id and amino acid length	correctly identifies at least three of the listed features	correctly identifies at least two of the listed features.	Wrong identification of all the listed features	no answer
Protein Function	All functions	most functions	Some functions	Wrong protein function identification	no answer
Family and Domain information	Correct protein family, conserved domain names, amino acid location	Correctly identifies two of the three required features	Correctly identifies one of the required three features	Wrong identification for all	no answer
Gene ontology information	Have correct six GO terms in three sections including id name and definition	Have correct six Go terms in the three sections but is missing the required description	Have no fewer than three correct GO terms with some description	Have fewer than three correct GO terms	no answer
Protein –protein interactions	Correctly Identifies all the three interactors with protein names and ids	Correctly identifies fewer three interactors but has the names and protein ids	Correctly identifies all the three interactors with missing names and ids	Identifies fewer than three interactors with no names and ids	No answer